Protocols
Reagent Protocols
User guide for using the Moxi Cyte Single Color Viability Reagent (Moxi V and Moxi GO II)
User guide for using the Moxi Cyte Two Color Viability Reagent (Moxi GO II only)
Moxi GO II
System-Related Protocols
Step-by-step procedure for swapping the 561nm/LP and 650nm/LP filters on the Moxi GO II
Protocol for using the validation check beads to ensure the Moxi GO II system is functioning properly
Cell Staining Protocols
Nuclei or Nucleated Cell Counting Protocol with Coulter Principle and Propidium Iodide
Step by step instructions for antibody labeling and detection of cell surface markers with the Moxi GO II System
Step by step instructions for intracellular immunolabeling of peripheral blood using the Moxi GO II system.
Step by step instructions for cell surface immunolabeling of peripheral blood using the Moxi GO II system.
Step by step instructions for staining with Calcein-AM and PI using the Moxi GO II system.
Step by step instructions for staining with FITC- Annexin V and PI using the Moxi GO II system.
Step by step instructions for staining with AO and PI using the Moxi GO II system.
Step by step protocol for processing whole blood for measuring mitochondrial potential using TMRE on the Moxi GO II system.
Step by step protocol for processing whole blood for ROS detection on the Moxi GO II system.
Moxi V
System-Related Protocols
Protocol for using the system validation check beads to ensure the Moxi V system is functioning properly
Cell Staining Protocols
Protocol for staining cells with Propidium Iodide (PI) for Viability Assessment
Rapid Protocol for determining the total nucleated count (TNC) in RBC contaminated samples
Moxi Z
System-Related Protocols
Protocol for using the validation check beads to ensure the Moxi Z system is functioning properly
Cell Counting Protocols
Step by step instructions for counting white blood cells (WBCs) using the Moxi Z system.
General Protocols
Step by step protocol for fixing cells in Ethanol before counting with any Moxi system.
Protocol for lysing red blood cells (RBCs) prior to counting with any Moxi instruments