Protocols

Reagent Protocols

User guide for using the MoxiCyte Single Color Viability Reagent

Moxi GO II

Instrument-Related Protocols

Step-by-step procedure for swapping the 561nm/LP and 650nm/LP filters on the Moxi GO II

Protocol for using the system validation check beads to ensure the Moxi GO II instrument is functioning properly

Cell Staining Protocols

Nuclei or Nucleated Cell Counting Protocol with Coulter Principle and Propidium Iodide

Step by step instructions for antibody labeling and detection of cell surface markers with the Moxi GO II System

Step by step instructions for intracellular immunolabeling of peripheral blood using the Moxi GO II system.

Step by step instructions for cell surface immunolabeling of peripheral blood using the Moxi GO II system.

Step by step instructions for staining with Calcein-AM and PI using the Moxi GO II system.

Step by step instructions for staining with FITC- Annexin V and PI using the Moxi GO II system.

Step by step instructions for staining with AO and PI using the Moxi GO II system.

Step by step protocol for processing whole blood for measuring mitochondrial potential using TMRE on the Moxi GO II instrument.

Step by step protocol for processing whole blood for ROS detection on the Moxi GO II instrument.

Moxi V

Instrument-Related Protocols

Protocol for using the system validation check beads to ensure the Moxi V instrument is functioning properly

Cell Staining Protocols

Protocol for staining cells with Propidium Iodide (PI) for Viability Assessment

Rapid Protocol for determining the total nucleated count (TNC) in RBC contaminated samples

Moxi Z

Instrument-Related Protocols

Protocol for using the system validation check beads to ensure the Moxi Z instrument is functioning properly

Cell Counting Protocols

Step by step instructions for staining with Annexin V (Apoptosis) and PI (Viability) using the Moxi Flow device.

General Protocols

Step by step protocol for fixing cells in Ethanol.

for Moxi instruments