Next Generation Cell Analysis with
Unparalleled Quality and Opportunities
- Sensitivity and Data Comparable to a Flow Cytometer
- Counting Thousands of Cells is More Accurate Than Imaging
- Obtain Accurate Counts Instead of Relative Estimates
- Differentiation of Cell Types in a Mixed Population
- Superior Characterization With Particle Size Resolution to 0.1µm
- Coulter Principle Counting = Greater Reproducibility With CVs <5%
- Multiplex Assays Feasible Using Additional Fluorescence Options
- Fast Time to Result: <15 seconds Per Sample
- 21 CFR Part 11 Software Available for Compliance
- Economical Price Affords Standardization Across Departments
- Power of a Flow Cytometer at a Fraction of the Cost
- Simple User Interface Usable by Any Lab Personnel
- Zero Instrument Maintenance, Cleaning, or Preparation Needed
- Biohazard-free Operation Using Disposable Sample Holder
- Small Instrument Footprint: <10 square inches
Instrument Overview
The Reliability of Coulter-Based Counting in the Palm of Your Hand
Love our cell counting technology but don’t need any fluorescence? Then our Moxi Z is the right machine for you. All the power of our microfluidics-based cell counting literally in the palm of your hand, and at a low, cost-effective rate. It provides the same robust cell counting technology of the Moxi Go II and Moxi V to make sure you are getting the true cell counts with every run.
Unbeatable Viability Counting
Don’t need two fluorescence channels, but want some fluorescent capabilities for viability measurements? We have you covered with our single 561nm/LP (Orange/Red) cell viability instrument in the Moxi V. Moxi V provides true cell viability counts, with 50-100x more cells counted compared to a vision counters. Get precise cell characterization with CVs less than 5%, without the need for triplicates and a self-contained, easy to use, and affordable machine.
The Power of a Small-Scale Flow Cytometer in a Square Foot of Space
With our Moxi Go II system, we have coupled our cell counting technology with the ability to detect two channels of fluorescence to revolutionize the way that cell counting is done. Our 488nm laser and Photo Multiplier Tubes (PMTs) gives the accuracy and detection capabilities of a normal flow cytometer for both a 525/45nm (Green) channel and 561nm/LP (Orange/Red) channel. This combination gives you unparalleled bench top power when working with any cell types marked with any of your favorite fluorescent cell markers. At the price of a single years’ service contract for a normal flow cytometer, unlock the next stage of cellular analysis with first-in-class technology at a fraction of the cost for traditional cell analysis methods!
Applications Overview
The flexibility provided by Moxi instruments allows for an unprecedented number of applications. Reliable Coulter-based counting and precise measurements provides for high quality PBMC analysis from any blood sample with any of our Moxi instruments. Flow cytometry-like sensitivity from the Moxi GO II gives the ability to detect antibody-labeled cells, which primes it for use in any workflow that involves immunoprofiling like CAR-T therapies, iPSC-based gene therapy, single cell genomics, and more. Additionally, the fluorescence capabilities of the Moxi V and Moxi GO II enables robust one or two-color viability measurements with either our proprietary viability reagents or with your own favorite cellular stains.
How it Works
ORFLO’s proprietary cell counting technology can count tens of thousands of cells in seconds, quickly providing best-in-class data. Normally, accuracy is negatively impacted by acquisition time. However, by leveraging the current gold standard Coulter Principle, we bring you both with no compromises to either. All of this in a self-contained, easy-to-use, and benchtop-sized instrument. Throw in the option for PMT-based fluorescence detection, and the possibilities become limitless.
The core of our technology lies in what is most commonly referred to as the Coulter Principle. Put simply, we are using a gap between two electrodes to measure the change in voltage as a cell passes through it.
Through this change in voltage, we can accurately determine the volume and diameter of the cell that passed through (Detectable size range = 3µm-26µm for S/S+ cassettes; 4µm-35µm for M/M+ cassettes). Because of the precise nature of the Coulter Principle and our ability to monitor minute changes in electrical current rapidly and in real time, thousands of cells can be passed through the aperture and recorded/sized within seconds. These thousands of instances are placed into scatter plot that makes it easy to see the cells present in your sample and manipulate the data on our touch-screen interface.
We have revolutionized the industry by miniaturizing this Coulter Principle-based counting into a small disposable microfluidics cassettes. We have created a simple, effective, and self-contained way to get everything you need, with all of the speed and none of the bulk of other cell counters or flow cytometers. Just insert the cassette, pipette in a small amount of your precious sample, and let the microfluidics and our proprietary cell counting technology do the rest.
With our Moxi GO II system, we have coupled our cell counting technology with the ability to detect two channels of fluorescence. Our 488nm laser and dual Photo Multiplier Tubes (PMTs) give the accuracy and detection capabilities of a normal flow cytometer for both a 525/45nm (Green) channel and 561nm/LP (Orange/Red) channel. This combination gives you unparalleled benchtop power when working with any cell type marked with any of your favorite fluorescent cell markers.
The Moxi V, while only having a single fluorescence channel and lower sensitivity, utilizes identical algorithms to ensure that counting and fluorescence data is captured and analyzed simultaneously.
