Coulter Principle Cell Counting
Do you need to count tons of cells every single day, with utmost precision?
Get gold standard accuracy with the Moxi instruments that use the Coulter Principle
How It Works
ORFLO’s proprietary cell counting technology can count tens of thousands of cells in seconds, quickly providing best-in-class data. Normally, accuracy is negatively impacted by acquisition time. However, by leveraging the current gold standard Coulter Principle, we bring you both with no compromises to either. All of this in a self-contained, easy-to-use, and benchtop-sized instrument. Throw in the option for PMT-based fluorescence detection, and the possibilities become limitless.
The Coulter Principle
The core of our technology lies in the ideas developed and refined by Wallace H. Coulter in the 1940s, most commonly referred to as the Coulter Principle. Put simply, we are using a gap between two electrodes to measure the change in voltage as a cell passes through it.
Through this change in voltage, we can accurately determine the volume and diameter of the cell that passed through (Detectable size range = 3µm-26µm for S and S+ cassettes; 4µm-35µm for M and M+ cassettes). Because of the precise nature of the Coulter Principle and our ability to monitor minute changes in electrical current rapidly and in real time, thousands of cells can be passed through the aperture and recorded/sized within seconds. These thousands of instances are placed into a histogram or scatter plot that makes it easy to see the cells present in your sample and manipulate the data on our touch-screen interface.
Microfluidics Meets Cell Counting
Don’t like wasting half of your sample on figuring out whether your experiment worked correctly? Don’t like spending time and money on a large-scale flow cytometry run for a simple transfection efficiency or cell viability check? Our innovative microfluidics cassettes have created a simple, effective, and self-contained way to get everything you need, all for a low cost of as little as 15µL. Just insert the cassette, pipette in a small amount of your precious sample, and let the microfluidics and our proprietary cell counting technology do the rest.
Dual-Channel PMT Fluorescence: (Moxi GO II)
With our Moxi GO II system, we have coupled our cell counting technology with the ability to detect two channels of fluorescence, revolutionizing the way that cell counting is done. Our 488nm laser and dual Photo Multiplier Tubes (PMTs) give the accuracy and detection capabilities of a normal flow cytometer for both a 525/45nm (Green) channel and 561nm/LP (Orange/Red) channel. This combination gives you unparalleled benchtop power when working with any cell type marked with any of your favorite fluorescent cell markers.
Why is the Coulter Principle crucial for scientists?
A gold standard principle for automated cell counters
Analyze thousands of cells in seconds
Selectively count cells of different sizes
Check out our other cell counters that use the Coulter Principle
Don’t need two channels, but want some fluorescent capabilities? We have you covered with our single-channel cell viability instrument, the Moxi V
Love our cell counting technology but don’t need any fluorescence? Then our Moxi Z is the right machine for you. All the power of our microfluidic cell counting literally in the palm of your hand, and at a low, cost-effective rate.
