Next Generation Cell Analysis with
Moxi Instruments

High Quality Data

Fast, Easy, and Secure Results

Sensitive Fluorescence Detection

  • Counting Thousands of Cells is More Accurate Than Imaging
  • Fast Time to Result: <15 seconds Per Sample
  • Sensitivity and Data Comparable to a Flow Cytometer
  • Obtain Accurate Counts Instead of Relative Estimates
  • 21 CFR Part 11 Software Available for Compliance
  • Differentiation of Cell Types in a Mixed Population
  • Superior Characterization With Particle Size Resolution to 0.1µm
  • Simple User Interface Usable by Any Lab Personnel
  • Multiplex Assays Feasible Using Additional Fluorescence Options
  • Coulter Principle Counting = Greater Reproducibility With CVs <5%
  • Zero Instrument Maintenance, Cleaning, or Preparation Needed

Instrument Overview

Moxi Z

Moxi V

Moxi GO II

Next Generation
Coulter Counter

Next Generation
Cell Analyzer

Next Generation
Mini Flow Cytometer​

No Fluorescence

1 Channel
Fluoresence

2 Channel
Fluoresence

The Reliability of Coulter-Based Counting in the Palm of Your Hand​

Unbeatable Viability Counting

The Power of a Small-Scale Flow Cytometer in a Square Foot of Space

Applications Overview

The flexibility provided by Moxi instruments allows for an unprecedented number of applications. Reliable Coulter-based counting and precise measurements provides for high quality PBMC analysis from any blood sample with any of our Moxi instruments. Flow cytometry-like sensitivity from the Moxi GO II gives the ability to detect antibody-labeled cells, which primes it for use in any workflow that involves immunoprofiling like CAR-T therapies, iPSC-based gene therapy, single cell genomics, and more. Additionally, the fluorescence capabilities of the Moxi V and Moxi GO II enables robust one or two-color viability measurements with either our proprietary viability reagents or with your own favorite cellular stains.

 

General Immunoprofiling

Can Be Done On:

CAR-T

Can Be Done On:

Single Cell Genomics

Can Be Done On:

PBMC Analysis

Can Be Done On:

Cell Viability

Can Be Done On:

How it Works

ORFLO’s proprietary cell counting technology can count tens of thousands of cells in seconds, quickly providing best-in-class data. Normally, accuracy is negatively impacted by acquisition time. However, by leveraging the current gold standard Coulter Principle, we bring you both with no compromises to either. All of this in a self-contained, easy-to-use, and benchtop-sized instrument. Throw in the option for PMT-based fluorescence detection, and the possibilities become limitless.

The core of our technology lies in what is most commonly referred to as the Coulter Principle. Put simply, we are using a gap between two electrodes to measure the change in voltage as a cell passes through it.

Through this change in voltage, we can accurately determine the volume and diameter of the cell that passed through (Detectable size range = 3µm-26µm for S/S+ cassettes; 4µm-35µm for M/M+ cassettes). Because of the precise nature of the Coulter Principle and our ability to monitor minute changes in electrical current rapidly and in real time, thousands of cells can be passed through the aperture and recorded/sized within seconds. These thousands of instances are placed into scatter plot that makes it easy to see the cells present in your sample and manipulate the data on our touch-screen interface.

We have revolutionized the industry by miniaturizing this Coulter Principle-based counting into a small disposable microfluidics cassettes. We have created a simple, effective, and self-contained way to get everything you need, with all of the speed and none of the bulk of other cell counters or flow cytometers. Just insert the cassette, pipette in a small amount of your precious sample, and let the microfluidics and our proprietary cell counting technology do the rest.

With our Moxi GO II system, we have coupled our cell counting technology with the ability to detect two channels of fluorescence. Our 488nm laser and dual Photo Multiplier Tubes (PMTs) give the accuracy and detection capabilities of a normal flow cytometer for both a 525/45nm (Green) channel and 561nm/LP (Orange/Red) channel. This combination gives you unparalleled benchtop power when working with any cell type marked with any of your favorite fluorescent cell markers.

The Moxi V, while only having a single fluorescence channel and lower sensitivity, utilizes identical algorithms to ensure that counting and fluorescence data is captured and analyzed simultaneously.